The regenerative properties of the extracted polysaccharide from Brittle star (Ophiocoma erinaceus) on cutaneous wound in male Wistar rat

نویسندگان

  • Elaheh Amini Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Iran
  • Ilnaz Abolhasani Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
  • Javad Baharara Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
  • Naser Mahdavi Shahri Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده مقاله:

In traditional medicine, extracts of polysaccharide-containing plants are widely employed for the treatment of cutaneus wounds. The brittle star, as one of marine organisms, encompasses bioactive compounds, which confer the healing potency of damaged arms. The aim of the present study is to investigate the effects of extracted polysaccharide from the Persian Gulf brittle star )Ophiocoma erinaceus( on male Wistar rat skin wound healing. In this experimental study, 60 male Wistar rats were divided randomly into 5 groups including control, positive control (treatment with honey), experimental 1 to 3 (treatment with 12.5, 25, 37.5 mg/kg body weight of extracted polysaccharide, respectively). In all groups, the wound was inflicted in the posterior part of the body of rats. Then, rats were treated locally. On the 3th, 7th, 10th and 14th days, samples were collected from the healing hole and histological changes were investigated by light microscopy. Then, quantitative data were analyzed by SPSS software, one-way ANOVA at the level of p<0.05. Microscopic results showed that, in treated wounds by 37.5 mg/kg of extracted polysaccharide, as well as positive control, the re-epithelialization, the angiogenesis and the attenuation of inflammatory cells were significantly improved in comparison with the control group. Maximum epithelium thickness (on the 7th day) and minimum inflammatory cells (on the 14th day) were observed in the experimental group 3 (treated with 37.5 mg/kg of extracted polysaccharide) and positive control, as compared with the control group (p≤0.05). The findings of this research indicated that the polysaccharide extracted from O. erinaceus accelerated the cutaneus wound healing in male rats, which can be used as a natural component in the development of natural source agents for the treatment of cutaneous wounds.  

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Metastatic Inhibitory and Radical Scavenging Efficacies of Saponins Extracted from the Brittle Star (Ophiocoma erinaceus).

Echinodermata use saponins in chemical defense against pathogens and predators. The molecular mechanisms of antimetastatic effects of brittle star saponins are still unknown. The present study examined antioxidant capacity and invasive ability in HeLa carcinoma cells exposed to brittle star crude saponins. Discolorating methods with DPPH and ABTS and expression of SOD-2 with RT-PCR were used to...

متن کامل

Anti-inflammatory properties of saponin fraction from (Spiny brittle star) Ophiocoma erinaceus (Müller and Troschel, 1842)

Marine saponins are naturally glycosides compounds which possess important biomedical properties such as anti-cancer, antioxidant and anti-inflammatory. Nevertheless, no systematic studies on the anti-inflammatory potential of brittle star saponin and its underlying mechanisms have been reported. In this study, Ophiocoma erinaceus extracted saponin was investigated for its in vitro anti-inflamm...

متن کامل

Hemolytic and cytotoxic effects of saponin like compounds isolated from Persian Gulf brittle star ( Ophiocoma erinaceus )

Hemolytic and cytotoxic effects of saponin like compounds isolated from Persian Gulf brittle star (Ophiocoma erinaceus) Elaheh Amini*, Mohammad Nabiuni, Javad Baharara, Kazem Parivar, Javad Asili Developmental Biology, Kharazmi University, Tehran, Iran Biology, Research Center For Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Cell and Developmental Biology, Science and...

متن کامل

The in vitro and in silico Anticholinesterase Ac-tivities of Brittle Star (Ophiocoma erinaceus) crude venoms from the Persian Gulf-Bushehr

Background: Brittle stars can produce well-known toxins responsible for various of their biological activities in addition to their physical defense. The purpose of this study was to identify secondary metabolites and assess the anticholinesterase activities of the crude venom of the Persian Gulf Ophiocoma erinaceous (brittle star) in vitro and in silico due to the abundance of this marine life...

متن کامل

the effect of traffic density on the accident externality from driving the case study of tehran

در این پژوهش به بررسی اثر افزایش ترافیک بر روی تعداد تصادفات پرداخته شده است. به این منظور 30 تقاطع در شهر تهران بطور تصادفی انتخاب گردید و تعداد تصادفات ماهیانه در این تقاطعات در طول سالهای 89-90 از سازمان کنترل ترافیک شهر تهران استخراج گردید و با استفاده از مدل داده های تابلویی و نرم افزار eviews مدل خطی و درجه دوم تخمین زده شد و در نهایت این نتیجه حاصل شد که تقاطعات پر ترافیک تر تعداد تصادفا...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 7  شماره 1

صفحات  9- 18

تاریخ انتشار 2020-04

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023